SA, “no see-um” is actually a misquote of “No See’m technology”, a technique that has been in use for over a decade:
https://www.google.com/search?q=No+See’m+technology
Systematic Assembly and Genetic Manipulation … – NCBI – NIH
https://www.ncbi.nlm.nih.gov › articles › PMC7120124
by EF Donaldson · 2008 · Cited by 6 — Key Words: MHV, coronavirus, murine, infectious clone, No See’m technology, site-directed mutagenesis, type IIs restriction enzymes, reverse genetics.
Abstract · Introduction · Materials · Methods
Systematic Assembly of a Full-Length Infectious cDNA of …
https://www.ncbi.nlm.nih.gov › articles › PMC136593
by B Yount · 2002 · Cited by 351 — Consequently, its function in MHV replication and pathogenesis is not clear. … clones should remain in the assembled product (No See’m technology).
Abstract · MATERIALS AND METHODS · RESULTS · DISCUSSION
Systematic Assembly and Genetic Manipulation of the Mouse …
https://experiments.springernature.com › articles
Engineering mutations with the No See’m approach: (A) The position of … coronavirus, MHV, No See’m technology, type IIs restriction enzymes …
(PDF) Development of mouse hepatitis virus and SARS-CoV …
https://www.researchgate.net › publication › 8119695_De…
“no-see-um” technology allows for the assembly of large DNAs from smaller sub-. clones without the incorporation of unique restriction sites into the genome …
